Of course, speaking about visual methods, we mean that you will see not a separate organism, but a cluster of them. Nephelometric estimation method, globally speaking, based on measuring the turbidity of a substance, the more turbid is the solution, the more bacteria are there. Direct a beam of light at it on one side, and on the other, install a device that catches the transmitted light. Now take the cloudy solution of interest to us with the bacteria, and we let’s do the same with it. In which case will the device catch more light?
Using the nephelometric method, If we compare the obtained data with the previously obtained standart curve, we can find out the number of bacteria per ml.